1. Expression of functional eGFP-fused antigen-binding fragment of ranibizumab in Pichia pastoris

Shirin Movaghar Asareh , Tahereh Savei , Sareh Arjmand* , Seyed Omid Ranaei Siadat* , Fataneh Fatemi, Mehrab Pourmadadi, Javad Shabani Shayeh

BioImpacts. 2022;12(3): 203-210. doi: 10.34172/bi.2021.23219

PMCID: PMC9124873 PMID: 35677669 Scopus ID: 85132073917

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Recombinant heavy and light chains of anti-VEGF-A monoclonal Ab Fab fragment were produced in Pichia pastoris. Reconstruction of Fab fragments was verified by anti-Fab Ab and a split system. An electrochemical setup was used to test the interaction of the reconstructed Fab fragment with VEGF-A.

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